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Title Oral biology : molecular techniques and applications / edited by Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng.
Imprint New York : Humana Press : Springer, [2017]
©2017

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Edition Second edition.
Description 1 online resource (xiv, 503 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1537
Methods in molecular biology (Clifton, N.J.) ; v. 1537. 1064-3745
Bibliog. Includes bibliographical references and index.
Note Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK). WlAbNL
Available only to authorized UTEP users.
Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL
Subject Mouth -- Microbiology.
Dentistry -- Research.
Mouth -- microbiology.
Periodontal Diseases -- microbiology.
Genre Laboratory Manual.
Contents Salivary diagnostics using purified nucleic acids / Paul D. Slowey -- RNA sequencing analysis of salivary extracellular RNA / Blanca Majem, Feng Li, Jie Sun, and David T.W. Wong -- Qualitative and quantitative proteome analysis of oral fluids in health and periodontal disease by mass spectrometry / Erdjan Salih -- Antioxidant micronutrients and oxidative stress biomarkers / Iain L.C. Chapple, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, and Melissa M. Grant -- NMR-based metabolomics of oral biofluids / Horst Joachim Schirra and Pauline J. Ford -- Gene therapy of salivary diseases / Bruce J. Baum, Sandra Afione, John A. Chiorini, Ana P. Cotrim, Corinne M. Goldsmith, and Changyu Zheng -- Oral microbiota in health and disease : an overview of molecular findings / José F. Siqueira Jr. and Isabela N. Rôças -- Microbial community profiling using terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE) / José F. Siqueira Jr., Mitsuo Sakamoto, and Alexandre S. Rosado -- Analysis of 16S rRNA gene amplicon sequences using the QIIME software package / Blair Lawley and Gerald W. Tannock -- Adhesion of yeast and bacteria to oral surfaces / Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, and Ann R. Holmes -- Quantitative analysis of periodontal pathogens using real-time polymerase chain reaction (PCR) / Ma José Marin, Elena Figuero, David Herrera, and Mariano Sanz -- Methods to study antagonistic activities among oral bacteria / Fengxia Qi and Jens Kreth -- Natural transformation of oral Streptococci by use of synthetic pheromones / Gabriela Salvadori, Roger Junges, Rabia Khan, Heidi A. Åmdal, Donald A. Morrison, and Fernanda C. Petersen -- Markerless genome editing in competent Streptococci / Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Åmdal, Fernanda C. Petersen, and Donald A. Morrison -- Tools and strategies for analysis of genome-wide and gene-specific DNA methylation patterns / Aniruddha Chatterjee, Euan J. Rodger, Ian M. Morison, Michael R. Eccles, and Peter A. Stockwell -- Generating multiple base-resolution DNA methylomes using reduced representation bisulfite sequencing / Aniruddha Chatterjee, Euan J. Rodger, Peter A. Stockwell, Gwenn Le Mée, and Ian M. Morison -- Protocol for the determination of the methylation status of gingival tissue DNA at specific CpG islands / Trudy J. Milne -- Genome-wide analysis of periodontal and peri-implant cells and tissues / Moritz Kebschull, Claudia Hülsmann, Per Hoffmann, and Panos N. Papapanou -- Differential expression and functional analysis of high-throughput -omics data using open source tools / Moritz Kebschull, Melanie Julia Fittler, Ryan T. Demmer, and Panos N. Papapanou -- Exploring genome-wide expression profiles using machine learning techniques / Moritz Kebschull and Panos N. Papapanou -- Embryonic explant culture : studying effects of regulatory molecules on gene expression in craniofacial tissues / Katja Närhi -- Oral epithelial cell culture model for studying the pathogenesis of chronic inflammatory disease / Mike R. Milward, Martin R. Ling, Melissa M. Grant, and Iain L.C. Chapple -- Fabrication and characterization of decellularized periodontal ligament cell sheet constructs / Amro Farag, Cédryck Vaquette, Dietmar W. Hutmacher, P. Mark Bartold, and Saso Ivanovski -- Method to isolate, purify, and characterize human periodontal ligament stem cells / Krzysztof Mrozik, Stan Gronthos, Songtao Shi, and P. Mark Bartold -- Constructing tissue microarrays : protocols and methods considering potential advantages and disadvantages for downstream use / Lynne Bingle, Felipe P. Fonseca, and Paula M. Farthing -- Growing adipose-derived stem cells under serum-free conditions / Diogo Godoy Zanicotti and Dawn E. Coates -- Quantitative real-time gene profiling of human alveolar osteoblasts / Dawn E. Coates, Sobia Zafar, and Trudy J. Milne -- Proteomic analysis of dental tissue microsamples / Jonathan E. Mangum, Jew C. Kon, and Michael J. Hubbard -- Characterization, quantification, and visualization of neutrophil extracellular traps / Phillipa C. White, Ilaria J. Chicca, Martin R. Ling, Helen J. Wright, Paul R. Cooper, Mike R. Milward, and Iain L.C. Chapple.
Summary This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.
Other Author Seymour, Gregory J., editor.
Cullinan, Mary P., editor.
Heng, Nicholas C. K., editor.
Other Title Print version: Oral biology. New York : Humana Press, ©2017 9781493966837