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Title Breast cancer : methods and protocols / edited by Jian Cao.
Imprint New York, NY : Humana Press, 2016.


 Internet  Electronic Book    AVAILABLE
Description 1 online resource (xii, 332 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1064-3745 ; v. 1406
Methods in molecular biology (Clifton, N.J.) ; v. 1406. 1064-3745
Note Includes index.
Bibliog. Includes bibliographical references and index.
Note Available only to authorized UTEP users.
Online resource; title from PDF title page (SpringerLink, viewed February 4, 2016).
Subject Breast -- Cancer -- Laboratory manuals.
Breast Neoplasms.
Biomarkers, Tumor.
Molecular Diagnostic Techniques.
Genre Laboratory Manual.
Laboratory manuals.
Electronic books.
Contents Basic histopathological methods and breast lesion lypes for research / Nengtai Ouyang and Lin Wang -- Clinical applications for immunohistochemistry of breast lesions / Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu -- Immunohistochemistry for triple-negative breast cancer / Kalnisha Naidoo and Sarah E. Pinder -- In situ hybridzation of breast cancer markers / Li Min and Chengchao Shou -- Evaluation of human epidermal growth factor receptor 2 (HER2) gene status in human breast cancer formalin-fixed paraffin-embedded (FFPE) tissue specimens by fluorescence in situ hybridization (FISH) / Harry C. Hwang and Allen M. Gown.
Quantification of mRNA levels using real-time polymerase chain reaction (PCR) / Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou -- Detection of miRNA in cultured cells or xenograft tissues of breast cancer / Martin Brown and Meiyun Fan -- Pyrosequencing analysis for breast cancer DNA methylome / Cem Kuscu and Canan Kuscu -- Vita-assay method of enrichment and identification of ciruclating cancer cells/circulating tumor cells (CTCs) / Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen -- Breast cancer stem cell isolation / Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell.
Cellular apoptosis assay of breast cancer / Yu Sun and Wei-Xing Zong -- Assessment of matrix metalloproteinases by gelatin zymography / Jillian Cathcart -- Assessment of synthetic matrix metalloproteinase inhibitors by fluorogenic substrate assay / Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang -- Determination of breast cancer cell migratory ability / David Schmitt, Joel Andrews, and Ming Tan -- A novel collagen dot assay for monitoring cancer cell migration / Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao -- Three-dimensional assay for studying breast cancer cell invasion / Nikki A. Evensen -- A combined phagocytosis and fluorescent substrate degradation assay to simultaneously assess cell migration and substrate degradation / Ashleigh Pulkoski-Gross -- Analysis of invadopodia formation in breast cancer cells / Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du -- Patient-derived tumor xenograft models of breast cancer cells using genetically encoded biosensors / Maryia Lu, Li Wei Rachel Tay, Jingquan He, and Guangwei Du -- 3D in vitro model for breast cancer research using magnetic levitation and bioprinting method / Fransisca Leonard and Biana Godin.
Methods for analyzing tumor angiogenesis in the chick chorioallantoic membrane model / Jaquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks -- Pharmacokinetics and pharmacodynamics in breast cancer animal models / Wei Wang, Subhasree Nag, and Ruiwen Zhang -- Intracellular delivery of fluorescently labeled polysaccharide nanoparticles to cultured breast cancer cells / Derek Rammelkamp, Weiyi Li, and Yizhi Meng -- Imaging matrix metalloporteinase activity implicated in breast cancer progression / Gregg B. Fields and Maciej J. Stawikowski.
Summary Annotation This volume provides resources, ideas, and bench manuals for the study of breast cancer. The book is divided into five sections: methods used in clinical laboratory for diagnosis; methods used in both clinical and research laboratories for testing genetic alterations; methods used to study the behaviour of breast cancer cells; and methods used for mimicking human breast cancer in a living organism.
Other Author Cao, Jian (Professor of Medicine), editor.
Other Title Print version: Breast cancer. New York, NY : Humana Press, 2016 1493934422 9781493934423