Limit search to items available for checkout
Title Xenopus protocols : post-genomic approaches / edited by Stefan Hoppler, Peter D. Vize.
Imprint New York : Humana Press, ©2012.


 Internet  Electronic Book    AVAILABLE
Edition 2nd ed.
Description 1 online resource (xiii, 568 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 917
Methods in molecular biology (Clifton, N.J.) ; v. 917. 1064-3745
Bibliog. Includes bibliographical references and index.
Note Available only to authorized UTEP users.
Online resource; title from PDF title page (Springer Protocols, viewed Oct. 26, 2012).
Subject Xenopus -- Embryology -- Laboratory manuals.
Xenopus -- Genetics -- Laboratory manuals.
Xenopus -- Eggs -- Laboratory manuals.
Xenopus -- Cytology -- Laboratory manuals.
Cellular signal transduction -- Laboratory manuals.
Genre Laboratory Manual.
Laboratory manuals.
Contents pt. I Xenopus tropicalis. Xenopus tropicalis as a model organism for genetics and genomics : past, present, and future / Robert M. Grainger -- Husbandry of Xenopus tropicalis / Alan Jafkins [and others] -- Generating diploid embryos from Xenopus tropicalis / Florencia del Viso and Mustafa Khokha -- Navigating the Xenopus tropicalis genome / Ira L. Blitz -- pt. II Genetics in Xenopus. Genetic analysis of Xenopus tropicalis / Timothy J. Geach, Derek L. Stemple, and Lyle B. Zimmerman -- Forward genetic screens in Xenopus using transposon-mediated insertional mutagenesis / Donald A. Yergeau [and others] -- Targeted gene disruption with engineered zinc-finger nucleases (ZFNs) / John J. Young and Richard M. Harland -- Reverse genetic studies using antisense morpholino oligonucleotides / Yanan Zhao, Shoko Ishibashi, and Enrique Amaya -- Chemical genetics and drug discovery in Xenopus / Matthew L. Tomlinson, Adam E. Hendry, and Grant N. Wheeler -- Maternal mRNA knock-down studies : antisense experiments using the host-transfer technique in Xenopus laevis and Xenopus tropicalis / David J. Olson, Alissa M. Hulstrand, and Douglas W. Houston -- pt. III Xenopus trangenesis. Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI) / Shoko Ishibashi, Kristen L. Kroll, and Enrique Amaya -- Simple Method of Transgenesis using I-SceI Meganuclease in Xenopus / Shoko Ishibashi, Nick R. Love, and Enrique Amaya -- Using (Phi)C31 Integrase to Mediate Insertion of DNA in Xenopus Embryos / You E. Li, Bryan G. Allen, and Daniel L. Weeks -- Xenopus Transgenics: Methods Using Transposons / Clair M. Kelley [and others] -- Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements / Hajime Ogino [and others] -- Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression / Daniel R. Buchholz -- pt. IV Gene expression: from gene to transcript to protein. Chromatin Immunoprecipitation Analysis of Xenopus Embryos / Robert C. Akkers, Ulrike G. Jacobi, and Gert Jan C. Veenstra -- Transcriptomics Using Next Generation Sequencing Technologies / Dasfne Lee-Liu [and others] -- Databases of Gene Expression in Xenopus Development / Michael J. Gilchrist and Nicolas Pollet -- Investigating Alternative RNA Splicing in Xenopus / Agnes Mereau and Serge Hardy -- Immunoisolation of Protein Complexes from Xenopus / Frank L. Conlon [and others] -- Complementary Proteomic Analysis of Protein Complexes / Todd M. Greco [and others] -- pt. V Imaging Xenopus development. Antibody Development and Use in Chromogenic and Fluorescent Immunostaining / Eamon Dubaissi [and others] -- Multiple Fluorescent In Situ mRNA Hybridization (FISH) on Whole Mounts and Sections / Robert Lea [and others] -- Methods to Analyze microRNA Expression and Function During Xenopus Development / Boyan Bonev and Nancy Papalopulu -- Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos / Helene Auger [and others] -- Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos / Sagar D. Joshi, Hye Young Kim, and Lance A. Davidson -- pt. VI Novel approaches in Xenopus. Mathematical Modeling of Gene Regulatory Networks in Xenopus Development / Yasushi Saka -- Stem-Cell-Like Embryonic Explants to Study Cardiac Development / Boni A. Afouda -- Studying Regeneration in Xenopus / Caroline W. Beck -- On-Line Resources for Xenopus / Jeff Bowes.
Summary During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.
Other Author Hoppler, Stefan, 1965-
Vize, Peter D.
Other Title Print version: Xenopus protocols. 2nd ed. New York : Humana Press ; Springer, ©2012 9781617799914